The species composition and distribution of seagrass species in Pulau Setindan has never been evaluated, unlike the other islands of Johor where studies on seagrasses were assessed more extensively. In the present study, field surveys were carried out to investigate the seagrass distribution and species composition on the coastal areas of Pulau Setindan during period of July to August 2018. A total of six species from five genera were recorded, namely Cymodocea rotundata, Cymodocea serrulata, Enhalus acoroides, Halodule uninervis, Halophila ova/is, and Thalassia hemprichii. Results also indicated that seagrass beds were patchy and in the form of mixed beds, with more than two species of seagrasses coexisting. The coverage area was more than 10 ha and was concentrated on the shallow flats connecting Tanjung Genting on the mainland to Pulau Setindan. The dominant species found is Cymodocea rotundata with total percent cover of29.77% and found in all sampling sites, followed by Cymodocea serrulata with total percent cover of 45 .17%, occurring in 3 sites. Overall, sea grasses at Pulau Setindan is significant from a biological point of view as it is the closest representation of an undisturbed seagrass area of a non-marine park island in Johor. Thus, this study forms an initial step in understanding the seagrass community and provides a basis for future observations on the marine ecosystem of Pulau Setindan