Introduction: Stroke is one of the most recognisable diseases affecting the Malaysian population. The growing incidence of a stroke every year requires nursing students to get their knowledge updated. Objective: To provide a baseline on our nursing student’s level of knowledge and information needs on stroke to prepare them as future healthcare providers in the community. Methods: A cross-sectional study was conducted using adapted questionnaires to assess the level of knowledge and information needs of stroke among 30 nursing students from Kulliyyah of Nursing, International Islamic University Malaysia through a convenience sampling method. Descriptive statistics were used in data analysis via SPSS version 27.0. Results: The mean age of the students was 23.23 (±0.68), 96.7% were female and residents of Mahallah Ummu Kalthum, all of them were year four students, and 93.3% were single. Almost 43.3% had a family history of stroke while 50% have experience in taking care of stroke patients. The results highlighted that nursing students have good stroke knowledge with a mean score of 13.17 (±2.20) but a moderate score for information needs on stroke [125.83 (±15.62)]. Conclusion: Overall, nursing students showed that they have better knowledge regarding stroke disease but require further information on stroke-related nursing skills. This is in lieu of the diversity of stroke and its subtype which affect different nursing care needed.