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Successful preservation of banana Embryogenic callus by minimal growth And cryopreservation technique
Ika Roostika1, Fitri Damayanti2, Witjaksono3.
This paper reports successful preservation methods by minimal growth and
cryopreservation of banana embryogenic callus. The minimal growth and
cryopreservation techniques can be applied for medium-term and long-term
preservation. The embryogenic callus of banana cv Dwarf Parfitt was used as
the plant material. Different kind of medium formulations, temperature, gelling
agents, and ABA concentrations were tested in minimal growth study. The type
of explants, dehydration periods, and preculture media were tested in
cryopreservation study. The result showed that the use of WIC medium was
better than BM2G for maintaining embryogenic callus. The application of 0.005
mg L-1 ABA combined with reduced temperature (150C) could preserve the
cultures longer than 4 months with high survival rate (95%), high recovery rate
(90%), and high number of recovered embryos indicating high embryogenic
potential. For cryopreservation study, the best dehydration period was 20
minutes. The combined treatments of 150 g/l sucrose and 2 M glycerol at
preculture step was the most suitable treatment for successful
cryopreservation with 80% survival rates; and the recovered cultures
maintained their somatic embryogenic capacity.
Affiliation:
- Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Bogor 16111, Indonesia, Indonesia
- Universitas Indraprasta PGRI, Faculty of Math and Science,13760, Jakarta, Indonesia, Indonesia
- Research Center for Genetic Engineering, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Jl. Raya-Jakarta Km 46, Cibinong, Bogor 16911, Indonesia, Indonesia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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6 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (1.4) |
Rank |
Q3 (Engineering (all)) |
Additional Information |
SJR (0.191) |
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