This research study aims to investigate the effectiveness of utilizing Synthesia Artificial Intelligence (AI), an advanced instructional tool, in enhancing laboratory experiments. The research focused on evaluating the impact of incorporating Synthesia AI as a supplementary learning resource on student learning outcomes in laboratory settings. Synthesia AI is a cutting-edge technology that revolutionizes students’ immersive learning experience. With its advanced machine learning algorithms, Synthesia AI can generate photorealistic video content featuring virtual actors and presenters that can be programmed to speak in multiple languages, accents, and even emotional tones. In this study, an instructional-based laboratory experiment was conducted using Synthesia AI. The participants were from the Sabah Branch of UiTM, and enrolled in a Basic Plant Science laboratory course under the Pre-Diploma Agrotechnology programme. The course was divided into two groups, an experimental group and a control group. The experimental group received laboratory instructions through Synthesia AI videos, while the control group received traditional laboratory instructions. The quantitative data were collected through pre and post-tests to assess the student’s learning outcomes. The qualitative data were obtained through a survey containing open-ended questions to gather the students’ feedback on their experience with Synthesia AI videos. The experimental group, which received laboratory instructions through Synthesia AI videos, was expected to perform better in the post-test than the control group. The findings of this research have provided valuable insights into the potential benefits and challenges of integrating Synthesi IO as an instructional tool, 6 thereby contributing to the enhancement of laboratory-based instruction. With its ability to create realistic virtual simulations, Synthesia AI could significantly enhance laboratory education, allowing for more cost-effective, innovative and accessible teaching methods, and ultimately empowering the next generation of scientists and innovators.