Due to its vast application in life sciences,mcell culture is one of the modes of choice for research and has been used extensively in antibody, vaccine and hormone production. Techniques in cell culturing are traditionally learnt hands-on in the laboratory setting. Instructors demonstrate the cell culturing techniques for students to emulate or re-perform. However, the lack of trained instructors, limited number of equipment and increased costs are major hindrances in hands-on learning of cell culture techniques. Online learning was initially necessitated for instructors by the unprecedented event of COVID-19 pandemic worldwide which led to the limitations in conducting practical and hands-on sessions. In this review, various teaching and learning methods which include blended learning, virtual laboratory, or massive online open course (MOOC), and assessment modalities related to online teaching of cell culture techniques are discussed. The benefits of these modalities to students and instructors such as increased motivation and more engaged learning are discussed. Even though the benefits of these modalities are substantial, we also discuss the limitations related to online learning such as less interpersonal communication and inability to nurture all the senses that usually happens in hands-on learning. Suggestions on the advancement and improvement of such modalities are also discussed in this review. The present review will aid educators and instructors in planning, designing modules and executing online teaching for cell culture techniques.