Epoxidized natural rubber/ polylactic acid blends with catappa fibre filler were prepared by melt blending followed by compression molding to fabricate test samples. The effect of fibre loading which is catappa fibre on physical and mechanical properties of biocomposite ENR/PLA/KTP were investigated. This research was to study the optimum percentage of catappa fibre besides to prove that catappa fibres exhibit antibacterial property. The characterization carried out throughout the study was to investigate the capability of biocomposite to be used as insole application. The tensile strength was optimum at 15% fibre loading while the modulus strength increased with increasing fibre loading. Impact strength decreased with high fibre content. Morphology examination showed good fibre dispersion in the matrix at 15% KTP fibre. Percentage of water absorption was observed to increase when fibre loading was increased. The antibacterial test showed positive results whereby biocomposite ENR/PLA/KTP showed inhibition activity towards Gram positive bacteria (Staphylococcus aureus) which is the common bacteria can be found in shoe insoles.