Plutella xylostella, diamondback moth (DBM), has been one of the most challenging insects to control in the world to date. Environmentally friendly methods of control, such as the use of botanical insecticides, are available. A formulation that can sustain the main compound’s level is required, which can be accomplished through soluble powder nanoformulation. The goal of this research was to test and evaluate the ability of clove oil nanoparticles produced from polyethylene glycol 6000 (PEG 6000) to control DBM utilizing a solid dispersion technique. Bioassay by leaf dip method in laboratory was used to test the lethal effect of clove oil nanoparticles (CO-NPs) on DBM. Clove oil nanoformulation was successful because it produced nanoparticles (179.98 nm in diameter) while maintaining high levels of the active component eugenol. Clove oil nanoparticles may increase clove oil’s toxicity to DBM, seen from the LC50 value after 24 h of treatment. The LC50 values for clove oil nanoparticles after 24 and 48 h of treatment were 10.308 and 9.451%, respectively.