Introduction: Proton magnetic resonance spectroscopy (1H-MRS) is an advanced magnetic resonance imaging (MRI) technique that permits the molecular characterization by detecting signals of the water, lipids, and metabolites such as lactate, N-acetyl aspartate (NAA), glutamine, creatinine (Cr), and choline (Cho) in the region of interest. 1H-MRS technique has widely explored the area in the brain, breast, prostate, and optical radiation. However, there are lim- ited literature on MR spectroscopy techniques in musculoskeletal (MSK). The primary purpose of this pilot study is to evaluate lower limb muscles strength pre-and post-resistance straining in lightly active subjects using single-voxel 1H-MRS with different voxel sizes. Methods: The study examined the vastus lateralis muscle of nine male adults 18- 26 years of age, body mass index (BMI) = 21.9-38.0 kg/m2. Each subject underwent 1.5 T single voxel, short echo time, point resolved proton MRS at pre- and post- resistance training. Results: The Wilcoxon signed-rank test was performed and was found no significant difference between 20 mm2 and 30mm2 voxel size for NAA, Cr, and Cho for pre- and post-resistance training. Conclusion: The finding of this study shows no improvement of the metabolite’s quantification from two different sizes of a voxel. However, it may be helpful to explore a different aspect of tech- nique in 1H-MRS imaging to investigate the muscle size, volume, and musculoskeletal properties with significant conditions such as musculoskeletal diseases, muscle injuries as well as in sports sciences.