Introduction: The COVID-19 pandemic has had a big impact on fishermen’s lives. Fishermen, as informal workers, are vulnerable to a decline in the quality of life. By default, fishermen’s quality of life is considered lower than other professions before the COVID-19 pandemic happened. Now, their quality of life had become even lower during the pandemic. In relations to this, the objective of this study was to compare fishermen’s quality of life before and during the COVID-19 pandemic. Methods: The study was conducted among 70 fishermen in Kenjeran using a simple random sampling technique. The data was obtained using a questionnaire which comprised of sociodemographic characteristics and quality of life measurement using the Quality of Life BREF (WHOQOL-BREF). The fishermen’s quality of life was measured based on physical, psychological, social, and environmental dimensions. The data was then analyzed using descriptive statistics and the Wilcoxon Signed Rank test. Results: The results showed significant decline in the mean scores of the quality of life in the total and all quality-of-life dimensions. Conclusion: The results of this study can be used to determine adaptation strategies for fishermen to improve their quality of life.