Introduction: During the recent COVID-19 pandemic situation, teachers are more likely to experience job stress due to an imbalance between job demands and the ability to manage those demands. The purpose of this study was to determine factors related to job stress among public high school teachers in the Jakarta Pusat region during the COVID-19 pandemic. Methods: Data were collected from 113 public high school teachers in 2021 during the second wave of COVID-19 using the NIOSH Generic Job Stress Questionnaire (GJSQ). Results: Findings showed that 47,8% of teachers experience job stress. It was found that role conflict (P .127), workload (P .134), and social support (P .056 were not significantly associated with job stress. Meanwhile, role ambiguity (P .015) was significantly associated with job stress. Conclusion: These findings suggest role ambiguity of the public-school teachers during the COVID-19 pandemic becomes the major factor associated with job stress.