African catfish (Clarias gariepinus) is in high demand in the Malaysian market. However, the species is known to be easily infected with diseases such as fungi, due to poor water quality and bad handling, which can cause mortality and economic losses. In addition, fungal infection in fish eggs can reduce their production. Four African catfish were purchased from local markets in Padang Hiliran, Kuala Terengganu and Kampung Manir, Kuala Terengganu, Terengganu, Malaysia. The objective of this study was to isolate and identify fungi from infected African catfish and their eggs. Affected fish with symptoms such as a swollen lesion, irregular swimming, and dark discolouration were screened for pathogenic fungi. Isolation, identification, and extracellular enzymatic assays were performed for the isolated fungi. Potato dextrose agar was used as an isolation medium for isolating fungi on African catfish and eggs. Fungi were identified by macroscopic observation of growth morphology and their structure, followed by microscopic observation after staining with lactophenol cotton blue to observe hyphae. Three fungi were isolated and identified as Mucor sp. (n=2) and Trichoderma sp. (n=1). All isolated fungi were also analyzed for extracellular enzymes, such as proteases, amylases, and lipases, to test their pathogenicity. Trichoderma sp. showed positive results in all tests. Moreover, a strain of Mucor sp. reacted positively to the amylase and lipase test while the other strain of the same species showed no reaction, indicating a negative result. In conclusion, isolated fungi have virulent properties and can cause mortality if the body and eggs are infected. Therefore, fish farms need comprehensive health management to prevent the spread of fungal diseases.