This study aimed to assess the knowledge and practices of nurses regarding infection control at HMC Hospital. A questionnaire-based survey was conducted among 156 participants, focusing on various aspects of infection control. The results revealed that a significant proportion of nurses were using medical masks (79%) and following color-coding segregation for biomedical waste disposal (81%). However, a lower percentage of nurses were using eye protection (43%) and recapping needles after use (76%). The findings indicated the need for improvement in adherence to infection control practices, particularly in the areas of personal protective equipment usage and safe needle handling. Additionally, a high level of awareness was observed regarding the importance of hand hygiene and biomedical waste generation hazards and legislation, with 79% and 75% of nurses acknowledging their significance, respectively. The study highlights the importance of consistent adherence to infection control practices and emphasizes the need for targeted interventions to enhance knowledge and promote best practices among nurses at HMC Hospital. By addressing the identified gaps, healthcare facilities can strengthen their infection control measures and contribute to improved patient safety and reduced healthcare-associated infections.