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Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms
Aqeel, Moaz1, Bukhari, Sayed Muhammad Ata Ullah Shah2, Ullah, Hidayat3, Hussain, Tahir4, Ullah, Aman5, Jamal, Muhsin6, Nawaz, Muhammad Asif7, Andleeb, Saadia8, Khan, Khurshaid9, Pervez, Sidra10, Imran, Muhammad11.
Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the known remedies seem ineffective. Bacteriophages as a natural killer of bacteria can offer alternative therapy for antibiotics. The study aimed to isolate and characterize a lytic bacteriophage against P. aeruginosa (clinically resistant strain/superbug) and to investigate its lytic potential to control bacterial planktonic cells and their biofilms on stainless steel surfaces. A lytic bacteriophage known as MAC-1 was isolated from wastewater against the selected P. aeruginosa-2750 strain. Its host range was moderate and it only infected six isolates. Phage MAC-1 was characterized through transmission electron microscopy and was classified to the Siphoviridae family. It has good heat and pH stability. Its latent time was 24 min with a burst size of about 410 virions per cell. The phage MAC-1 efficacy was determined against P. aeruginosa planktonic cells and biofilms on metallic surfaces. Isolated phage MAC-1 demonstrated promising activity against bacterial planktonic cells as well as in reducing bacterial biofilm biomass formed in 96-well and on stainless steel plates. However, a phage cocktail may be used to avoid resistance and ensure complete eradication of bacterial biofilms.
Affiliation:
- National University of Sciences and Technology, Palau
- National University of Medical Sciences, Pakistan
- Abdul Wali Khan University, Pakistan
- Abdul Wali Khan University, Pakistan
- National University of Medical Sciences, Pakistan
- Abdul Wali Khan University, Pakistan
- Shaheed Benazir Bhutto University, Pakistan
- National University of Sciences and Technology, Palau
- Abdul Wali Khan University, Pakistan
- Shaheed Benazir Bhutto Women University, Pakistan
- University of Health Sciences, Pakistan
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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6 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Web of Science (SCIE - Science Citation Index Expanded) |
Impact Factor
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JCR (1.009) |
Rank |
Q4 (Multidisciplinary Sciences) |
Additional Information |
JCI (0.15) |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (1.4) |
Rank |
Q2 (Multidisciplinary) |
Additional Information |
SJR (0.251) |
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