A survey of fish diseases in tiger grouper Epinephelus fuscoguttatus at two sites of nursery cages in Sabah was conducted during June-December 2009. From the examination of 90 and 60 juvenile tiger groupers from Tanjung Badak and Pulau Layang-Layang nursery cages respectively, 5 taxa of parasites (Trichodina, Benedenia, Caligus, Pseudorhabdosynochus and unidentified metacercariae); 2 viruses (VNN and iridovirus) and 10 bacterial groups (Staphylococcus, Vibrio, Photobacterium, Non-fermentor, Micrococcus, Pseudomonas, Gemella, Erwinia, Cedecia and Aerococcus) were recorded. This study highlighted the prevalence of pathogen in nursery cages of tiger grouper cultured in Sabah waters.