Conventional way of measuring glucose level using finger pricking method does not only cause pain but is also costly to diabetic patients since the lancet and test strip is not reusable. Addressing to this matter, a simple metabolic heat conformation (MHC) technique was adapted in our study to measure human glucose level using non-invasive method which is harmless and capable of providing real time monitoring. This method is adapted based on the theory of glucose metabolism process which produces adenosine triphosphate (ATP). ATP function to transport energy within cells in form of heat dissipated throughout human body. Thus, temperature based algorithm was conducted using an implemented prototype circuit sensor which consist of pyroelectric detector and NTC thermistor to detect small variation of human and surrounding temperature. A total of 50 samples have been collected for analysis purposes. Results obtained from the temperature-based prototype glucose monitoring system using NTC thermistor (TPGMS-NTC) were compared with commercial automated glucose analyzer through Error Grid Analysis, and it was shown that glucose concentration is correlated to the total amount of heat dissipated from human body by 0.9125. About 90% of the samples taken are plotted within accurate range (region A) while 10% are plotted within acceptable range (region B).