Wound healing is a natural restorative response to tissue injury. Nowadays, wound healing study has become an active area for many researchers and the development of biomaterials as wound management product has been increased. Biocompatibility is one of the most important criteria in biomaterials selection. The objective of this study is to examine the cytotoxicity effect of newly developed chitosan derivatives film on fibroblasts growth and its proliferationin-vitro. The effect of oligochitosan on the secretion of interleukin 8 (IL-8) and transforming growth factor beta (TGF-β) also is being evaluated. The cytotoxicity of these chitosan products were compared by measuring cell survival with the tetrazolium salt reduction (MTT) assay which measured the mitochondrial activity of active living cells. The determination of fibroblasts proliferation was done by using CellTiter 96® Aqueous Non- Radioactive Cell Proliferation Assay. The results demonstrated that oligochitosan film was non-toxic. Low molecular weight chitosan derivatives have the ability to induce the proliferation of fibroblasts and stimulation of proliferation increased with time exposure. However, no fibroblasts proliferation detected in the presence of high molecular weight chitosan derivatives during the 5 days of incubation. The secretion of IL-8 by fibroblasts was also detected at low concentration of chitosan derivatives. However, none of TGF-β was detected. This study clearly demonstrated the growth modulating effects of chitosan on fibroblasts in-vitro. Thus, further study should be developed in order to clarify the effect of biomedical grade chitosan for wound management applications.