The present study aims at determining the reliability and validity of self-administered Affective Disturbance and Distress Scale among working women in Pakistani service organizations. In this regard, questionnaires were distributed among 60 lecturers and doctors. Initially reliability statistics were calculated, which showed that Cronbach’s alphas ranged from 0.71 to 0.81, whereas inter scale correlations ranged from 0.59 to 0.77, thus confirming the reliability of scale. On the other-side, content validity of scale was established through experts or field researchers in the university. Construct validity of scale was checked by Exploratory Factor analysis, which showed that the factor loadings ranged from 0.84 to 0.91. The overall results showed that the Affective Disturbance and Distress scale demonstrated good psychometric properties following its administration among the Pakistani working women. Therefore, it can be utilized as a reliable and valid self-administered instrument for assessing the mental health of working women in Pakistan.