In this study, the performance and emission characteristics were analyzed by using rubber biodiesel in a single cylinder direct injection diesel engine. The experiments were conducted using different combination of fuels such as 20%, 50% of biodiesel blends by volume (B20 and B50) with pure diesel fuel, pure biodiesel (B100) and pure diesel fuel (B0). The performance parameters were obtained for different load conditions from No load to Full load at rated rpm. Results indicated that the higher brake thermal efficiency, Mechanical efficiency, reduced specific fuel consumption obtained for biodiesel blend of B20, compared to other blends and diesel fuel.