This paper shows the determination of iodine value (IV) of pure and frying palm oils using Partial Least Squares (PLS) regression with application of variable selection. A total of 28 samples consisting of pure and frying palm oils which acquired from markets. Seven of them were considered as high-priced palm oils while the remaining was low-priced. PLS regression models were developed for the determination of IV using Fourier Transform Infrared (FTIR) spectra data in absorbance mode in the range from 650 cm-1 to 4000 cm-1. Savitzky Golay derivative was applied before developing the prediction models. The models were constructed using wavelength selected in the FTIR region by adopting selectivity ratio (SR) plot and correlation coefficient to the IV parameter. Each model was validated through Root Mean Square Error Cross Validation, RMSECV and cross validation correlation coefficient, R2 cv. The best model using SR plot was the model with mean centring for pure sample and model with a combination of row scaling and standardization of frying sample. The best model with the application of the correlation coefficient variable selection was the model with a combination of row scaling and standardization of pure sample and model with mean centering data pre-processing for frying sample. It is not necessary to row scaled the variables to develop the model since the effect of row scaling on model quality is insignificant.