Reactive dyes are synthetic organic compounds widely used in several industries including textile, paper, printing and cosmetics, which have inverse effects on human life and environment. Reactive Black 5 (RB5) and Remazol Brilliant Blue R (RBBR) are type of reactive dye group that can decolorize by microorganisms. Three species of bacteria Brevibacillus panacihumi ZBI, Lysinibacillus fusiformis ZB2 and Enterococcus faecalis ZL and three species of fungi fungus Candida sp. S1, Meyerzoma sp. S7 and Rhizoctonia zeae SOL3 were used to decolorize dyes in individual and co-culture. Among the selected bacteria, Enterococcus faecalis ZL shows the highest decolorization rate of RB5 and RBBR reached to 77% and 58% respectively, in 4 days. Meanwhile, Rhizoctonia zeae SOL3 shows the highest decolorization ability among the selected fungi. The presence of glucose has a positive effect on decolorization by fungi and bacteria. In co-culture of Enterococcus faecalis ZL and Rhizoctonia zeae SOL3, the decolorization ability has decreased. This result suggests that fungi have adverse effect on the removal of dyes by bacteria.