The study of cancer cell has been hindered by the lack of appropriate ex vivo models, which can mimic this microenvironment. It is hypothesized that the fabrication of porous 3-D scaffolds for the biomimetics growth of cancer cells ex vivo could facilitate the study of the disease in its native 3-D niche. For that reason, biomaterials are used for fabrication of 3-D scaffold, in general, may be natural polymers such as proteins, collagens and gelatin, or synthetic biopolymers. Among the various available biodegradable polymers, polyhydroxyalkanoates (PHAs) have gained significant interest as one of the value-added materials which can be synthesized from abundantly available source of palm oil mill effluent (POME). Down the group of the PHA, poly-3-hydroxybutyrate (PHB) and copolymerizing this PHB that produced PHBVs; these two polymers have the most prevalent polymer used for scaffolds fabrication. A physico-chemical and biological modification has developed to improve wetting, adhesion, and printing of polymer surfaces, generally by introducing a variety of polar groups. These techniques must be tailored to introduce a specific functional group when the surface modification is a precursor to attach a bioactive compound. There are a few methods in order to fabricate porous 3-D scaffolds such as solvent casting, particulate leaching, thermally induced phase separation, gas forming, fiber bonding, electrospinning and also solid free form method. A review of the polyhydroxyalkanoates (PHAs) for tissue engineering applications is presented, beginning with the basic naturally derived polymerization of PHAs, biotransformation of palm oil mill effluent (POME) to the value-added polymers, novel methods of scaffold fabrication capabilities and its physicochemical and biological surface modifications to increase cell-biomaterial affinity.