Anterior cruciate ligament injury is extremely common in athletics and recreational sport. The emphasis of physiotherapy rehabilitation in post-ACL reconstruction is to enhance quality of life. The purpose of the study was to identify determinants of quality of life after ACL reconstruction rehabilitation. Sixty male patients who undergone 12 to 16 weeks of rehabilitation completed the background data, ACL quality of life (ACL-QOL), Lysholm, International Knee Documentation Committee (IKDC), Knee Injury and Osteoarthritis Outcome Score (KOOS) and Tanpa Scale Kinesiophhobia (TSK). Multiple linear regression showed that KOOS-pain and KOOSsport/recreation explained 59.5% of the variation in quality of life. Thus, pain and recreation were the determinants of quality of life. This finding is useful in identifying potential factors that enhance quality of life in ACL reconstruction rehabilitation.