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Identification of novel bacterial species capable of degrading Dalapon using 16S RRNA sequencing
Javad Hamzehalipour Almaki1, Rozita Nasiri2, Wong, Tet Soon3, Fahrul Zaman Huyop4.
2,2-dichloropropionic acid (2,2DCP) is used as herbicide in agricultural industry and it is one
of the halogenated organic compounds distributed widely in the world causing
contamination. In this study, a bacterial strain isolated from contaminated soil where
halogenated pesticides applied in Universiti Teknologi Malaysia and it was named “JHA1”.
Bacterium JHA1 was able to utilize 2,2 dichloropropionate 2,2-DCP or (Dalapon) as a source
of carbon and energy. Based on 16S rRNA analysis, the isolate showed 87% identity to
Terrabacter terrae strain PPLB. The identity score was lower than 98% so that it was
suggested to be new organisms that worth for further investigations if it will be proven that
this is novel. Therefore, current isolate was designated as Terrabacter terrae JHA1. The
isolate grew in the minimal media containing 10 mM, 15 mM, 20 mM and 25 mM of 2,2- DCP
as the sole energy and carbon source and the best growth rate was in 20 mM as the
optimum concentration of 2,2-DCP while bacterial growth was inhibited in medium with 30
mM 2,2-DCP.
Affiliation:
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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6 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (1.4) |
Rank |
Q3 (Engineering (all)) |
Additional Information |
SJR (0.191) |
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