The aim of this study was to determine the nutrient and anti-nutrient composition of six varieties of cassava leaf namely Medan, Sri Pontian, Putih, Kuning, Sri Kanji 1 and Sri Kanji 2. Proximate composition, minerals, cyanide and tannin content were analyzed. The results obtained showed that composition of nutrients and antinutrients in cassava leaves is different among the varieties. Cassava leaf of variety Medan has significantly high moisture content, while Sri Pontian has significantly high fibre content and Putih has significantly high content of protein compared to the other varieties. Highest ash content was found in both Putih and Sri Kanji 1 variety, while highest lipid was observed in both Sri Pontian and Sri Kanji 2. Both Medan and Sri Kanji 1 varieties showed significantly high in carbohydrate content. Among the varieties, Sri Pontian has the highest Ca content while Sri Kanji 1 has the highest Na content. Variety Kuning and Sri Kanji 1 have similarly high content of K, while Mg was found to be highest in both Medan and Sri Kanji 1 variety. For anti-nutrients composition, both cyanide and tannin were found to be significantly high in Sri Pontian variety compared to the other varieties. This study showed that cassava leaves has appreciable amount of nutrients and minerals that can be a good source of nutrients in a diet. However, proper pretreatments must be employed prior to consumption to reduce the anti-nutrients content in the leaves.