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A kinetic study of enzymatic hydrolysis of oil palm biomass for fermentable sugar using polyethylene glycol (PEG) immobilized cellulas
Umi Aisah Asli1, Isah Nwaha2, Hazirah Hamid3, Zainul Akmar Zakaria4, Aziatul Niza Sadikin5, Mohd Johari Kamaruddin6.
In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was
studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into
fermentable sugars as a feedstock for bioethanol production. The cellulase was
covalently immobilized with activated and functionalized polyethylene glycol (PEG)
via glutaraldehyde coupling method. As a whole, the immobilized cellulase displayed
50% higher efficiency over free cellulase, in reducing sugar recovery during hydrolysis
reactions at pH of 4.8 and temperature of 50ï‚°C. From the kinetic study, it showed that
Michaelis constant (Km) and limiting velocity (Vmax) of immobilized cellulase were 179.2
mg/ml and 33.5 mg/ml.min respectively, comparable with the value for free cellulose,
171.8 mg/ml and 34.5 mg/ml.min respectively. This result could be attributed to the
effect of PEG on the binding cellulase to substrate desorb substrates, and enables free
interaction of cellulase to hydrolyse cellulose maximally.
Affiliation:
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
- Universiti Teknologi Malaysia, Malaysia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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6 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (1.4) |
Rank |
Q3 (Engineering (all)) |
Additional Information |
SJR (0.191) |
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